Determination of the Membrane-Buffer Partition Coefficient with Laurdan Labeled Liposomes
نویسندگان
چکیده
منابع مشابه
Translational diffusion coefficient and partition coefficient of a spin-labeled solute in lecithin bilayer membranes.
The translational diffusion coefficient and the partition coefficient of a spin-labeled solute, di-t-butyl nitroxide, in an aqueous suspension of dipalmitoyl lecithin vesicles have been studied by electron spin resonance spectroscopy. When the lecithin is cooled through its phase transition temperature near 41 degrees C, some solute is "frozen out" of the bilayer, and the standard partial molar...
متن کاملApplication of Graph Theory: Relationship of Topological Indices with the Partition Coefficient (logP) of the Monocarboxylic Acids
It is well known that the chemical behavior of a compound is dependent upon the structure of itsmolecules. Quantitative structure – activity relationship (QSAR) studies and quantitative structure –property relationship (QSPR) studies are active areas of chemical research that focus on the nature ofthis dependency. Topological indices are the numerical value associated with chemical constitution...
متن کاملEXPERIMENTALl ANALYSIS OF PARTITION COEFFICIENT IN Al-Mg ALLOYS
Because the partition coefficient is one of the most important parameters affecting microsegregation, the aim of this research is to experimentally analyse the partition coefficient in Al-Mg alloys. In order to experimentally measure the partition coefficient, a series of quenching experiments during solidification were carried out. For this purpose binary Al-Mg alloys containing 6.7 and 10.2 w...
متن کاملDetermination of transmembrane pH gradients and membrane potentials in liposomes.
Techniques for determining large transbilayer pH gradients (delta pH) and membrane potentials (delta psi) induced in response to delta pH in large unilamellar vesicle liposomal systems by measuring the transbilayer redistribution of radiolabeled compounds have been examined. For liposomes with acidic interiors, it is shown that protocols using radiolabeled methylamine in conjunction with gel fi...
متن کاملDetermination of partition coefficient by the change of main phase transition.
The molar partition coefficients of amphiphilic additives, e.g. local anesthetics, between the aqueous phase, the liquid crystal and the gel phase of lipid membrane can be determined based on a combination of phase transition data obtained at high and low concentrations of the lipid in aqueous phase. The data obtained at high lipid concentration allow to find the phase diagram lipid-additive in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientia Pharmaceutica
سال: 2010
ISSN: 0036-8709,2218-0532
DOI: 10.3797/scipharm.cespt.8.pnm03